Good day everybody.
I am looking for a good advice what stereomicroscope is capable to detect GFP fluorescent through the Petri dish. My objects are calli, plantlets, etc in vitro, so i want them to stay sterile inside the dishes with media. Once I tried to make samples (my explants destroyed) and watch them with fluorescent microscope but autofluorescence level was too high.
So, is there the easy way to detect marker gfp? What is the solution in your lab?
We have used a NightSea adaptor that can be added to most stereoscopes. This option is much more cost effective if you have an existing stereoscope. We have used it to image calli. Here is the website- https://nightsea.com/products/stereomicroscope-fluorescence-adapter/ . You typically have to purchase them from EMS - https://www.emsdiasum.com/microscopy/products/magnifier/nightsea.aspx
We have success using this system.
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