I am blotting for a human truncated STAT3 (no N-terminus, no C-terminus). Looking through the biggest antibody supplier’s homepages, I only found antibodies specific for either the N or C-terminus of the protein. It does not matter if the antibody is poly- or monoclonal.
Dear Angelika. Perhaps this antibody would work for you? This has been validated by a fellow scientist from Aarhus University, Denmark:
http://pabmabs.com/wordpress/?p=1777
www.pAbmAbs.com is a website where scientists can share the experience regarding commercially available antibodies that they obtained through their research. I therefore hope you will share your experience as this would help others scientists that are also looking for suitable antibodies against these targets.
Kind regards,
Simon Molgaard
Although Simon's recommendation is fairly sound, my problem is the formulation of this product (good for 10 WBs at best). The product sheet does not say what part of the protein was used for the immunizing peptide, so it may not meet your requirements.
Considering the vast amounts of STAT3 antibodies on the market (1225 human STAT3 Western Blot Antibodies across 49 suppliers), it might be best to have an expert involved sorting the best products out from the market thus reducing the risks of spending fortunes on poorly or inconsistently performing antibodies.
Dear Simon, dear Jan,
Thank you for your comments - I did not know pAbmAbs before. In the meantime I found an antibody that worked for me from Millipore - 18F7.1. In Western blot, it only detects truncated recombinant Stat3 (no signal for truncated recombinant Stat1 or Stat5).
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