How to cite documents provided by an expert and industry standards?

Hi all How does one cite a document (not published) provided by an expert within the industry? The information in the document can be classified as best practice, and there exists little to no...

02 April 2020 3,552 3 View

What would be the best methodology to further explore the reasons behind a difference of rating on a Likert scale identified by weighted Kappa?

Would value colleagues opinions with a stronger qualitative background than mine as to what the optimum qualitative approaches be to try and answer the questions below: Our original thoughts...

17 November 2019 1,617 4 View

How much sample should be used in a no RT control?

I would like to know when running a no rt control for qPCR should the amount of template used be the same as the amount used to make cDNA or the amount of cDNA used in the qPCR. I use 100ng of RNA...

15 October 2018 7,022 3 View

Can anyone suggest the most appropriate analysis method for my data?

I have collected data in a classification task based on a 3 x 2 factorial design of categorical variables. Participants responded in eight separate tests each, and were asked to group three times...

20 February 2015 7,243 2 View

Are there blood tests that detect pancreatic cancer?

Detecting pancreas cancer

10 November 2013 6,113 0 View

Which variables are impertinent in irrigation scheduling when interfacing with arduino programming?

Arduino will be used as the micro-controller whereas android will be used as the monitoring device online.

04 July 2013 5,896 4 View

Paper not inclued

Title: Study of Reliable Seismic Data Communication Across Seismographic Stations: Case Study Nigerian National Network of Seismographic Stations (NNNSS) International Journal of Engineering...

01 January 1970 9,365 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View