How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

How to correctly reference instruments in systematic review following APA?

Hello, I am running into an issue and do not know how to cite / reference correctly here. APA states that instruments and measurements need to be referenced with the author's name. At the same...

11 February 2021 9,523 3 View

Why does the fatigue cracks initiate from surface to interior as the stress level decreases?

It is well known that low-cycle and high-cycle fatigue cracks initiate from the surface, while ultra-high cycle fatigue cracks initiate from the inside. I am very curious why this transformation...

17 January 2021 9,633 6 View

What is the reason for discontinued Stage II fatigue crack growth in pearlitic steel?

Conventionally, the process of Stage II fatigue crack growth is governed by Paris law with less microstructural effect. When fatigue crack growth test was carried out for pearlitic steels ( both...

03 January 2021 7,075 3 View

What is the correlation of stress level (in stress-controlled mode) for Indirect Tensile Fatigue Test (ITFT) with that of field load?

What is the correlation of stress level (in stress-controlled mode) for Indirect Tensile Fatigue Test (ITFT) with that of field load on pavement? I am trying to predict fatigue life of pavement...

24 December 2020 9,060 3 View

What are the assumptions and limitations of Miner's rule ? Is there any better cumulative damage model available for steel?

What are the assumptions behind miner's rule? Is there any cumulative damage model that takes care of these assumptions?

14 December 2020 333 1 View

B12 deficiency resistant to parenteral treatment ?

hello i have a 40 year old female patient with chronic fatigue and forgetfullnes who also has hashimatos disease wich is under control. Her serum B12 level vas 164 pg/ml. After treatment with a...

02 December 2020 6,676 3 View

How to simulate fracture in the axle housing ?

Hey there, I want to simulate & analyze the fracture and fatigue (crack growth) in the rear axle housing of heavy tracks (Volvo company) in Abaqus. My question is which method is suitable for...

12 November 2020 3,377 3 View

How can I classify Brain Waves on Python?

I'm working in my thesis project that's about how to avoid car accidents by body fatigue, I got some datasets where I got information abouth 12 person that had an EEG analisis when they were...

07 November 2020 4,845 2 View