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Does anyone have any idea as to why dansyl derivatives of hydroxyl groups appear to result in a 171 M/Z fragment while dansyl derivatives of amines tend to result in 170 M/Z fragments? In our...
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I am wanting to perform 2D gel eletrophoresis on treated 3D spheroids grown using a hanging drop plate and need to find out how much protein would be available in 12 day old spheroid cultures.
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