I'm trying to make a solution for haptoglobin measurement using this product. My method gives me the following directions:
0.6g o-dianisidine, 13g sodium phosphate monobasic and 0.5g EDTA dissolved in 1l deionised water, pH adjusted to 4.1.
I know that o-dianisidine is only slightly soluble in water (60mg/l) but possibly more soluble under acidic conditions however it still did not totally dissolve when at pH 4.1
If I dissolved in alcohol, approx (50ml) does anyone know if the addition of the alcohol to my buffer will affect the assay result ?
Anyone have experience with this colorimetric assay for haptoglobin? solving the same amount in