Does anyone use DSMZ institute's PCR method for deteting mycoplasma contamination in cell culture?

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Manuele Martinelli

Dear bkarti

give a look to this link

https://bitesizebio.com/23682/homemade-pcr-test-for-mycoplasma-contamination/

i used it to test my culture infected by micoplasma and after antibiotic treatment and it worked for me.

ciao

manuele

Govinda Sharma

We use this: https://www.sigmaaldrich.com/catalog/product/sigma/mp0025?lang=en®ion=CA