Does anyone use DSMZ institute's PCR method for deteting mycoplasma contamination in cell culture?

This method seems to be easy, sensitive and cost effective. All I am looking for internal control DNA. Also, please suggest any other easily available PCR methods or commercial kits

01 February 2019 9,048 2 View

How to detect biallelic mutation using T7 endonuclease assay?

I have transfected mouse ES cells with single gRNA, Cas9 system. I am able to detect cleaved bands after T7 endonuclease. However, how to determine if this is monoallelic or biallelic mutation?

11 December 2017 878 4 View

What should be the pulse length of electroporation for transfecting mammalian cells?

I am currently optimizing electroporation conditions for mouse embryonic stem cells. What should be ideal range of pulse duration?

06 July 2017 8,578 1 View

Is it a good idea to do an overlap extension PCR with KAPA polymerase?

I have tried amplifying my individual fragments using Phusion but it is not working consistently but always works fantastically with KAPA. I think I am unnecessarily wasting time on Phusion....

05 June 2016 9,302 4 View

What should be the ideal ratio of primer to template DNA in PCR?

I have tried doing a PCR with kapa as well as Phusion HF polymerase. I am getting amplification with kapa, but Phusion is just not working. Both the master mixes use same: 1.5mM MgCl2 and 0.2mM...

04 May 2016 6,915 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View