How anxiety can be separated from curiosity in open field test for mice?

It is seen that in open field test mice do rearing more suggesting they are more exploratory or curious. Now it is considered one of the markers of anxiety like behaviour. But it is curiosity I...

01 February 2019 1,157 2 View

Elevated plus maze VS. open field anxiety test?

For the detection of anxiety of mouse which test is more suitable and why? Thanks, MS

01 February 2019 2,780 1 View

Meaning of increased rearing and grooming?

I have found my mice showed more rearing and grooming but my question is why they do that more and what does it indicates? Thanks MS

01 February 2019 4,927 7 View

Hyperactivity in mouse?

How the hyperactivity of mouse can be measured and how we can understand that these mice are hyperactive? Thanks MS

01 February 2019 1,573 1 View

When is the best time to do mice behavior test?

Mice are naturally nocturnal but most of the lab behavioral tests are conducted in the day time. Is this confounding? Therefore when is the best time to do behavioral test of mice/rodents? Thanks...

31 December 2018 509 4 View

How many behavioral tests can be conducted in parallel in mice?

For some reasons most of the time multiple behavioral tests such as open field, rotarod, walking beam, and novel object recognition need to conduct in parallel. However, there may be time issue...

31 December 2018 8,055 3 View

Why oligodendrocytes behave differently in different parts of the CNS?

It is observed that oligodendrocytes functions differs based on its position in CNS, why?

31 December 2018 3,358 2 View

Which is better test to assess anxiety in mouse?

Dears, To test anxiety and locomotion commonly "Open filed" and "Elevated-plus maze" apparatus are used. However, for certain reasons multiple instruments is not possible to use in same study. To...

31 December 2018 4,901 4 View

Activator of B or T cells

31 December 2016 2,492 0 View

Antibody for CD8 T cells for immunohistochemistry/flurescence....

07 August 2016 6,347 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View