I’m working on cultivating retinal organoids, but it can’t success form eye field primordial clusters (EFPs) so far. Therefore, I would like to inquire if anyone has encountered a similar issue and could give me some advice.

The cell type used here is patient-derived iPSCs, which have undergone CRISPR-Cas9 editing to correct a mutation back to normal (wild type) form.

I use 2D to 3D retinal organoid differentiation protocol, so I don’t work with embryoid body. The picture below shows cells culture in adherent condition from Day 1 to Day 25. The result didn’t show any eye field primordial clusters (EFPs).

If someone has encountered this situation and is willing to provide advice, I would greatly appreciate it.

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