How to disaggregate country data into city ones?

I'm using a number of indicators in my research which are not available at a sub-national level. Is there a reliable method for disaggregating the country data into city ones? (preferably with a...

16 February 2021 6,861 3 View

Cross-lagged panel analysis and sample size?

I want to estimate the causal relationship between two latent variables over time using structural equation modelling. both latent variables are measured according to different indicators...

31 January 2021 3,777 5 View

IHC-paraffin experiment: how do you approach shopping for an antibody?

Hello everyone! I am shopping for an arginase 1 antibody to use for IHC-paraffin experiments with rat tissue. So far what I have understood is that if an antibody says it is reactive with rat,...

16 January 2021 794 3 View

SEM for panel data ?

I'm trying to find the effect of smart cities on sustainable development. I collected data for several indicators of smartness of a city for 15 cities over 12 years. seems like panel data (GMM...

12 January 2021 9,914 5 View

How to prepare H2O2 solution or certainly how can we prepare 30% w/v H2O2 solution?which solvent?

Inorder to enhance the photocatalytic activity of magnetic nano photocatalysts like zinc ferrite we can use H2O2.

10 November 2019 5,780 3 View

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18 May 2019 5,751 2 View

Can anyone suggest me to set up a suitable basis set for theoretical calculations of organometallic complex?

I have recently installed Gaussian 16 for dft calculations. I have no good idea about suitable basis set? How to specify basis set for heavy atom and light atoms? YouTube tutorial mainly related...

16 February 2019 9,237 4 View

What are the tips to be considered for peptide encapsulation in liposome?

I am working on nanoliposome formulation to encapsulate a synthetic peptide , I have tried many ways for encapsulation, but after dialysis there wasn't any peptide in liposomes which I analyzed by...

02 December 2018 8,010 7 View

How do I analyse my luciferase data?

Hi I'm looking for some help in analysing my luciferase data. I have a transcription factor and then a range of proteins I think will influence transcription factor activity. For each...

08 November 2018 4,617 3 View

Why is there a uniformly increasing stress-strain curve for some of the iron-based superalloys?

Hello to all the members of the world of science! It's a tensile-related question and kind of a basic one; but I'd really like to know the answer. So here we go: Notwithstanding the fact that for...

09 October 2018 8,970 4 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

What is the procedure to determine the permeability of concrete?

can anyone help me in this, it will help me with my research work

01 March 2021 834 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View