Does anyone know what the wash buffers (A and B) and Detection Stock are in Duolink kit?

09 September 2019 1 9K Report

We want to use Duolink by flow cytometry. We have the kit suitable for fluorescence, PLA probes, and primary antibodies, but we don’t have the wash buffers (A and B) and Detection Stock (5x). Is the Detection Stock needed for FACS experiments?

Valeria Tananska

Hi,

There is a recipe for wash buffers given in bio-protocol:

https://bio-protocol.org/e1479

For further advice on their proper use and on the detection reagent, have a look at this link:

https://www.sigmaaldrich.com/technical-documents/articles/biology/duolink-flow-troubleshooting.html

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