I'm trying to identify the effect of different cytokines on the level of F4/80 expression in macrophages from the peritoneum that may be produced when sterile inflammation is induced. I understand I will be checking in isolation and in vivo, as it maybe different.
chemokine release in the lavage and expression in the macros is something that you may want to check. MCP1, KC/Groa, the MIPs etc.
How about my personal favourite of 'sterile inflammation mediators': IL-33 (and other DAMPs released upon tissue damage).
In fact only the needle stimulus to obtain perotoneal exsudate can change de cytokyne profile. I use in control mice commercial saline to human injection and I observed low intracellular levels of TNF-alpha
I recomend you old paper: Ajuebor MN, Das AM, Virág L, Flower RJ, Szabó C, Perretti M. Role of resident peritoneal macrophages and mast cells in chemokine production and neutrophil migration in acute inflammation: evidence for an inhibitory loop involving endogenous IL-10J Immunol. 1999 Feb 1;162(3):1685-91.
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