At what time points should pro-inflammatory cytokines levels be tested in a mouse model of Prototheca algae infection?

I'm working on establishing an murine infection model of the Prototheca algae. Not much is known about the dynamics of the infection, nor the induced immune response. I'd like to use the...

19 October 2020 2,908 2 View

Why I observe the Si peak after analyzing PEO on EDX?

After putting the PEO coated glass substrate into the oven and subjecting it to a temperature of 180 degrees, it obtains a haze on the glass. A peak Si was observed after EDX analysis. Is there...

24 September 2020 6,335 6 View

Which reference gene would you recommend for qPCR analysis in mouse macrophages and epithelial cells?

Which gene would you recommend as reference in those types of cells? Until now, I was working with HPRT but it's not efficient for me anymore. Thank you for all of the answers!

22 September 2020 615 4 View

Meaning of first derivative dM/dH around magnetization reversal in magnetic nanoparticle systems?

When I calculate first derivative of M versus H curve measured for an ensemble of FM nanoparticles (below they blocking temperature) I obtain a very clear maximum, very close to the so called...

04 May 2020 5,116 4 View

IHC Andy Algorithm, how to use it?

Hello everyone! Probably, this issue was already raised, but I would really appreciate online help from someone who didi it. So - could anyone help me with fiji, Andy's algorithm? I try to run it...

26 January 2020 2,249 1 View

Can Alltech OA 1000 Organic acids column be used to analyse solutions in solvents such as DMSO, THF, butanol or ethanol?

I would like to study the dehydration of carbohydrates to HMF in other solvents than water, however I am not too sure if the HPLC column that we have is appropriate for analysing this type of...

03 April 2019 4,022 5 View

How to obtain correctly DC demagnetisation curve (for nanoparticles)?

How can I obtain correctly DC demagnetisation curve (for nanoparticles)? How should I specifically perform the measurements: a) Do I bring to saturation my sample once and then apply increasingly...

20 January 2019 8,441 3 View

How I know that structure is porous from XRD measurement?

In XRD results for porous boron-carbon samples I've got a huge signal (11 000 arb. units) for small angle at 2theta=2.7. I know that this signal can indicate the preaense of small particles or...

14 July 2018 9,366 15 View

How can I distinguish S. cerevisiae var. boulardii from other S. cerevisiae strains (preferrably by molecular methods)?

Does anybody know which molecular typing methods would be the best for checking if the strain of S. cerevisiae is boulardii or not? I can't determine microsatellites polymorphism due to a lack of...

02 April 2018 3,857 2 View

What is proper gating strategy for LIVEDEAD stained population in order to exclude dead cells from analysis?

Hi! I would like to discriminate dead cells from my population using LIVEDEAD fixable dye. In principle it stains amine residues in both live and dead cells, but because of cell membrane...

28 October 2017 5,109 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View