Dear Ankit,

you can't do it.

Usually 1X PBS buffer is a solution with a phosphate buffer concentration of 0.01M (if you buy it from most of company); then you start from a dilute solution and you want a more concentrated one. At this point you should prepare it ex novo.

Regards,

Claudia

Dear Ankit,

you can't do it.

Usually 1X PBS buffer is a solution with a phosphate buffer concentration of 0.01M (if you buy it from most of company); then you start from a dilute solution and you want a more concentrated one. At this point you should prepare it ex novo.

Regards,

Claudia

Dear Ankit, I recommend you to search e.g. Google for phrase [e.g. '0.1M solution of PBS'  or '0.1M solution of PBS AND 1XPBS']. There are a lot of results (1.840), explaining what 1XPBS is and what not. Therefore usually (as commercially available): 10X PBS = 0.1M. Cave: there are several recipes out for making "PBS" (which only means: "Phosphate Buffered Saline", not indicating which "ingredients= salts" the solution will have incorporated (e. g. Na-phosphate(s), K-phosphate(s), NaCl, KCl, Na2HPO4, KH2PO4 etc.).

For making 0.1M PBS, search for < how to make "0.1 M PBS" > and you'll get a lot of answers too. This said just to be practical in advice (knowing that making NOT PBS but usually Na-NA-PO4-buffers like Millonig's or even Na-cacodylate buffer for preparation purposes in SEM and/or TEM preparations.

Best wishes and good luck, Wolfgang

------------Note / COMMENT / STATEMENT added 26th of February 2016------------  This my comment has been visited by the author for the first time since it was posted in March or April  2013 and was corrected Fri, 26th of February 2016 only  regarding misspelling of 3 particular words.

Additionally I found out that my comment was downvoted 3 times without notification. I regret that the quality of my reply for 3 voters was unacceptable.

I thought first to delete the comment = Reply # 2  without substitution but found - in retrospective - that my original answer perhaps is / was a bit laborious but nothing was said wrongly.

The DOWNVOTERS*) (some of which might have been 'professional ones' as we / commenters personally are "hit" and exposed publicly sometimes for base motives, the latter might be understood by comparing the authors ResearchGate Score of 90 in 2013 with the recently achieved RG-Score of nearly 142 ) interestingly have overlooked the totally  incorrect answer of Antonios M. (see Re#07 below)  with regard to request of Zeravan M (Re#05):

from "Stock solution (0.1 M)" you can't make 1 M buffer solution.

I renounce to downvote that incorrect reply.

Regards, Wolfgang Muss

*) NB:  from other RG-members to which I am bound I often am informed that their replies or questions are downvoted with obviously perfidious motives. I can't add any more specific to this since downvotes are anonymous.

Dear Ankit, I wish this link will help you.

http://www.thelabrat.com/protocols/reagents.shtml

http://www.thelabrat.com/protocols/3.shtml

good luck

Googling the same question I have found that traditional 1X PBS solutions which are commercially available correspond to 0.01 M. So you have to order the 10X solution.

Following the same theory you could dissolve 1 tablet of PBS from Sigma in 20 ml of deionized water and then filter with 0.22 um filter to sterilize the solution and to remove possible debris.

hello every body could you please inform me how to make 10ml of 1M PBS solution ?

rule of proportion?

by the way.. https://www.elabprotocols.com/viewer/?id=234

You can make from scratch:

Stock solution (0.1 M)

Na2HPO4 (anhydrous): 12.0 g

NaH2PO4 · H20: 2.2 g

NaCl: 85.0 g

Distilled water: 1 liter

Hi Ankit

It would be really simple to use PBS tablets to prepare PBS solutions with different concentrations. you can find more information in this link

https://www.thermofisher.com/order/catalog/product/18912014

Good Luck,

Dear Babak,

the URL you provided unfortunately deals with 1xPBS only. Ankit needs instead (as of his request!) 10xPBS. It might be that there are sold PBS-buffer tablets for 10XPBS too.

Again I would like to explicitely state that for use in "SEM-tissue sample fixation" and / or preparations 'PS'(= 'phosphate' buffers) as well as other biological buffers might be suited better than PBS (but -yes - one can!).

I am glad that  Mozhdeh Ghani has upvoted your answer - and that I haven' downvoted your reply is confirmed by the fact that I definitely upvote your question which I couldn't do if I were 'your downvoter'....   Best wishes and regards, Wolfgang

How can we calculate the molarity of PBS solution, using some particular compound or all of them?

 Perhaps the anonymous Downvoter wants to donate some knowledge?

I am ready for being downvoted again...

Phosphate-Buffered Saline (PBS; 0.1 m, pH 7.4)

80 g NaCl

2.0 g KCl

21.7 g Na2HPO4 · 7H2O

2.59 g KH2PO4

Prepare in 1 L of ddH2O.

© 2014 Cold Spring Harbor Laboratory Press

Perhaps also consult: https://www.researchgate.net/post/What_is_the_difference_between_PBS_and_physiological_saline

Phosphate-Buffered Saline (PBS; 0.1 m, pH 7.4)

80 g NaCl

2.0 g KCl

21.7 g Na2HPO4 · 7H2O

2.59 g KH2PO4

Prepare in 1 L of ddH2O.

 Dear Ankit,

Are you sure you want PBS for fixation? Fixatives for electron microscopy are usually made in  0.1M phosphate buffer. Google Sorensen's phosphate buffer for a protocol, it is not the same as PBS and I understand it has a better buffering capacity than PBS and they can also differ significantly in osmolarity. 

For transmission electron microscopy it would be better to avoid phosphates which can cause precipitate artifacts and use cacodylate buffer or  PIPES or HEPES instead.

@Shahram NAZARI:

to be honest and to avoid therefore "very redundant" postings:

Instead your mentioning the "protocol / recipe" for PBS WITHOUT citing the corresponding reference  in your reply #14 you better should had recommended 

Pierre-Louis Tharaux's Reply  # 12, who proposed that at least 21 days before your reply. 

If you "copy and paste" ANYTHING from ANY source into ResearchGate-Threads it is not only welcome but IT IS EXPECTED that the corresponding source (also if this source is in RG-archive) is cited too.

Respectfully and cordially, Wolfgang