What can we do if a recombinant protein in GuHCL 8M precipitates when GuHCL is diluted?

Hello!. I need to remove a tail of solubility from S. aureus of a recombinant protein prior to Western blot, but when I am trying diluting the GuHCL 8M the protein precipitates. What can we do?...

02 February 2016 1,667 3 View