How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What kind of cell death is happening?

After 24 hours of treatment with plant extract, MCF-7 cells underwent this change. About 6 hours later, the cells are completely destroyed. I doubt what kind of cell death these cells have....

12 August 2020 5,345 3 View

Protocol for Inducing Necroptosis in Cell Culture ?

Hi I am looking for a protocol (with appropriate dilutions, concentrations, quantities) of inducing necroptosis in in vitro cell culture. I have found protocols using TNF alpha dosing and TAK1...

31 December 2019 5,798 3 View

Why is my protein expressed but not functional?

Hi guys, I am over-expressing the protein MLKL along with various tagged forms in an MLKL KO cell line. Despite being able to being able to visualize the protein by both immunofluorescence...

16 January 2019 6,587 5 View

Why is my protein expressed but not functional?

Hi guys, I am over-expressing the protein MLKL along with various tagged forms in an MLKL KO cell line. Despite being able to being able to visualize the protein by both immunofluorescence...

31 December 2018 2,725 6 View

Necroptosis Stuff - Constitutively active plasmids of RIPK3, RIPK1 and MLKL?

Hi, I would like to know if somebody is currently using expression plasmids of RIPK1, RIPK3 and MLKL? And if so, anyone has some mutants that leads to constitutively kinase activity of those,...

09 October 2018 3,830 0 View

Can DMSO be a positive control in a necroptosis assay?

Dear all, I would like to ask, if DMSO can be a positive control in this particular experience. L929 murine cells stimulated by murine-TNF-Alpha by 8 hours mainly leads to cell death by...

21 June 2018 2,136 2 View

Is it necroptosis or apoptosis?

I have perform Hypoxic treatment on AML-12 cells for 12hrs and there is significant up regulation of RIP3 in western blot and no expression of cleaved casp3. I did flow cytometry with PI annexin...

05 June 2018 7,978 5 View

Is it necroptosis or apoptosis?

I have perform Hypoxic treatment on AML-12 cells for 12hrs and there is significant up regulation of RIP3 in western blot and no expression of cleaved casp3. I did flow cytometry with PI annexin...

04 June 2018 2,608 4 View

Which are the optimal conditions for phospho-MLKL detection in Western Blot?

I have tried to detect phospho-MLKL in a Western Blot using the positive control cells L929 treated with 100 ng/ml TNF alpha, 100 nM Smac mimetic and 30 uM Z-VAD for 6h and then...

03 April 2018 5,697 1 View

Western blot markers for necrosis?

Is there an established protein marker for necrosis (but not necroptosis) or perhaps certain assays that should be done to directly determine necrotic cell death?

14 March 2018 184 4 View