Results on Parameter-Dependent Pareto Front?

Dear community, I would like to request some references related to results on parameter-dependent Pareto Front, if there are any. I am interested in studying the behavior of the Pareto Front with...

08 May 2019 9,669 3 View

How do I quantify cells and fibers in tissues used in immunohistochemistry?

Hi all, I would need to compare the number of cells and fibers in different part of a tissue which was used in fluorescent immunohistochemistry. However, I am not sure how to proceed. I could use...

21 March 2019 8,043 3 View

What Big Data solution use for deep learning on clinical data purpose ?

Hello everyone, I would like to perform machine learning and deep learning in a near futur on clinical data, coming from different sources and technologies (Omics data, Imaging data, and so...

23 August 2017 3,551 5 View

NLP : Short text classification/categorization

Hello everyones, I'm looking for a way to classify really short pieces of text (max 18 words) into 9 different classes. As a learning set, I've got a dataframe with each sentence (one column)...

12 July 2017 2,965 10 View

Change of metric and uniform continuity?

Dear all, we know, owing to a result appearing in the book of R. M. Dudley (Real analysis and Probability - Theorem 2.8.2), that for any separable metric space (S, d), there is a metric e on S,...

19 April 2017 4,159 6 View

Weak convergence of signed measures?

Dear all, I am searching a result concerning the weak convergence of signed measures defined on the Borel algebra of a given metric space. The result that i find so far is established for weak...

10 February 2017 2,604 3 View

Relation between $\mu$-continuity sets and the Borel sigma-algebra?

Dear all, For any sigma-finite measure $\mu$ on the Borel subsets of a metric space X (separable if needed), we recall that a $\mu$-continuity set is any Borel set B such that $\mu(boundary of B)...

23 January 2017 3,301 4 View

What is the dual space of bounded continuous functions defined on a metric space (not necessarily compact)?

What is the dual space of bounded continuous functions defined on a metric space (not necessarily compact)? (i would appreciate to have a reference for the result too)

16 January 2017 6,534 8 View

Has anyone noticed high or uneven fluorescence background with iBlot PVDF membrane stacks?

I was wondering if anyone else has used the iBlot PVDF membrane stacks and noticed uneven and/or relatively high autofluorescence / background when used for fluorescence Western blotting? I am...

29 May 2016 8,048 2 View

ELISA from HUVEC cell culture supernatant

I want to do an ELISA on HUVEC cell culture supernatant to check for TNF alpha release. Can anybody recommend a suitable cell density/ml medium? I am thinking to culture my cells in a 6 well...

17 February 2016 5,274 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View