In my experiment, I need to use ascorbic acid to treat cancer cells. As we all know ascorbic acid is very unstable in aqueous solution because of its oxidizing behavior, can anyone please tell me if I want to do treatment with ascorbic acid to cell lines then how could I prepare it? Should I adjust ascorbic acid to PH 7?
I think ascorbic acid must be prepared fresh and new, as it is affected a lot and is oxidized in aqueous solutions day after day. There are many suggestions you can see in the following link, which is entitled:
Can I store ascorbic acid stock in the freezer?
https://www.researchgate.net/post/Can_I_store_ascorbic_acid_stock_in_the_freezer
Tsao Li-Yi
I suggest you prepare fresh since it is a one step procedure. All you need to do is prepare the volume required per time, this you can calculate from your working protocol.
Hi,
I see you already got some answers and mine won't be very different. Ascorbic acid is very reactive and easily oxidised. You could freeze it if you put some nitrogen gas through it first. However that might be more work than preparing it each time fresh.
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