Does anyone know the approximate price for TruSeq on MiSeq?

More Woody Lin's questions See All

Can eQTL be validated by wet-lab experiments?

eQTL = Expression quantitative trait loci (eQTLs) are genomic loci that contribute to variation in expression levels of mRNAs. In other words, a SNP regulating RNA expression level of a gene. Is...

11 December 2017 4,776 3 View

Is there a quick assay that tells the rate of mutation accumulation of a cancer cell line in different time points?

Except using illumina's sequencing. I am looking for a cheap and quick essay that can estimate the rate of mutation accumulation.

02 March 2015 8,343 0 View

SEQC project for DNA-Seq to estimate the coverage for low abundant mutations - any thoughts?

Dear all, SEQC has published a nice paper to better improve our expectations about RNA-Seq data. I am wondering if SEQC would do a similar assessment for DNA-Seq such as somatic...

08 September 2014 2,265 2 View

Is the RNA degradation rate of frozen normal tissue in -80 much faster than tumor tissue?

I noticed that frozen normal tissues very often have much lower RIN values (from bioanalyzer) while matched tumor tissues still have pretty good RIN values. I suspect that it is because the...

07 August 2014 9,448 4 View

Where are the references of TTN (titin) mutations in hepatocellular carcinoma?

Dear all, I have been reading a review article published on Nature Review Clinical Oncology in 2013. In the paper entitled "Mutational landscape of HCC", I noticed that TTN was labeled as a gene...

06 July 2014 8,957 8 View

What can I do with Cancer Exome-Seq data without matched normal?

Dear scientists, I am aware that tumor exome-seq data without a matched normal will have difficulty for distinguishing somatic and germline mutations. I aim to find "important" somatic mutations...

06 July 2014 9,809 7 View

Is there a method to extract tumours and adjacent normal tissues from mice multiple times?

I am wondering if there is a method for dissecting adjacent normal and tumor samples from living mice without sacrificing the mouse? I hope to study the tumor evolution at multiple time points...

04 May 2014 4,825 4 View

Would RNA quality/quantity be good enough for sequencing when the liver tumor samples are frozen and sliced?

One of my colleague thinks that the RNA quality should be fine for those cryo-sliced liver tumor samples. We just need to use more sliced samples to get enough RNA. Is this correct?

03 April 2014 9,370 5 View

Where do I find the number of common genes between two species?

In trees of life, people have been using the degree of conservation to assign the branches. I am wondering where I can look up for the numbers representing the diversity or commonality between two...

10 November 2013 5,162 6 View

Why are hemoglobin genes very often downregulated in cancers?

I found that mRNA levels of hemoglobin genes are downregulated very often in cancers (attached figure). I have checked Google and Wikigene, but I couldn't find any review article giving it an...

10 November 2013 8,314 8 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Casey M Turk

I believe this is something you might have to call/email sales to find out...unless someone else has done this recently. They would be the ones with the most up to date information. http://www.illumina.com/company/contact_us.ilmn

Woody Lin

Hi Casey,

I have found out the price from sales. Thanks!

Woody