Is there a method to extract tumours and adjacent normal tissues from mice multiple times?

More Woody Lin's questions See All

Can eQTL be validated by wet-lab experiments?

eQTL = Expression quantitative trait loci (eQTLs) are genomic loci that contribute to variation in expression levels of mRNAs. In other words, a SNP regulating RNA expression level of a gene. Is...

11 December 2017 4,776 3 View

Is there a quick assay that tells the rate of mutation accumulation of a cancer cell line in different time points?

Except using illumina's sequencing. I am looking for a cheap and quick essay that can estimate the rate of mutation accumulation.

02 March 2015 8,343 0 View

SEQC project for DNA-Seq to estimate the coverage for low abundant mutations - any thoughts?

Dear all, SEQC has published a nice paper to better improve our expectations about RNA-Seq data. I am wondering if SEQC would do a similar assessment for DNA-Seq such as somatic...

08 September 2014 2,265 2 View

Is the RNA degradation rate of frozen normal tissue in -80 much faster than tumor tissue?

I noticed that frozen normal tissues very often have much lower RIN values (from bioanalyzer) while matched tumor tissues still have pretty good RIN values. I suspect that it is because the...

07 August 2014 9,448 4 View

Where are the references of TTN (titin) mutations in hepatocellular carcinoma?

Dear all, I have been reading a review article published on Nature Review Clinical Oncology in 2013. In the paper entitled "Mutational landscape of HCC", I noticed that TTN was labeled as a gene...

06 July 2014 8,957 8 View

What can I do with Cancer Exome-Seq data without matched normal?

Dear scientists, I am aware that tumor exome-seq data without a matched normal will have difficulty for distinguishing somatic and germline mutations. I aim to find "important" somatic mutations...

06 July 2014 9,809 7 View

Would RNA quality/quantity be good enough for sequencing when the liver tumor samples are frozen and sliced?

One of my colleague thinks that the RNA quality should be fine for those cryo-sliced liver tumor samples. We just need to use more sliced samples to get enough RNA. Is this correct?

03 April 2014 9,370 5 View

Does anyone know the approximate price for TruSeq on MiSeq?

For example, stem cell panel contains 100 genes. How much would it cost for getting the 100 genes from 100 samples? Price of Targeted RNA Fixed Panels of Illumina TruSeq.

11 December 2013 6,156 2 View

Where do I find the number of common genes between two species?

In trees of life, people have been using the degree of conservation to assign the branches. I am wondering where I can look up for the numbers representing the diversity or commonality between two...

10 November 2013 5,162 6 View

Why are hemoglobin genes very often downregulated in cancers?

I found that mRNA levels of hemoglobin genes are downregulated very often in cancers (attached figure). I have checked Google and Wikigene, but I couldn't find any review article giving it an...

10 November 2013 8,314 8 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Would you like to join our meta analysis research team?

I am excited to announce an opportunity for dedicated researchers to join our dynamic team for an ongoing meta-analysis study. If you are passionate about research and have a keen interest in...

01 August 2024 6,737 1 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Maurizio Pea

I do not know if my advice will worthwhile. However, good research

dctd.cancer.gov/.../DrHollingshead090507.pps

Typical Rodent Sample Collection Methods

http://dctd.cancer.gov/MajorInitiatives/Sep0507Phase0Workshop/DrHollingshead090507.pps

Woody Lin

It doesn't seem to be a valid URL. Thanks!

Thanks! It is what I am looking for!

Henry Lopez

I think by multiple sampling you will introduce too many variables, e.g. inflammasomes...., would healing event..