I'm measuring MMP9 in urine samples by using ELISA method. As the ELISA measures proenzyme and it's active form, to separate these forms usually uses geletin zymograghy. It would be appreciated if anyone knows the studies in which are compared the results of these methods.
Roopali Roy and Marsha Moses did this work. It is in their papers. You can try J Clin Oncol 2009 for references. We also used FRET substrates with urines, and others are using the FRET substrates now with PrAMA. You can look up papers by myself and Miles Miller, and Roopali and Marsha Moses.
In Roopali and Marsha's older papers, they used zymography to discover MMP9 and ADAM 12 in urines from breast cancer patients.
Unfortunately, zymography is not a good way to screen samples.
Thank you Marcia for your comment but I need a study to compare the results of an ELISA with those of zymography to show the rate of the corelation.
Dear Alireza
Please use the following articles:
- Mannello, Ferdinando, et al. "Silicate increases the release of MMP-9 forms in peripheral blood: why gelatin zymography differs significantly in citrate plasma and serum obtained with or without clot activators." Clinical chemistry 53.11 (2007): 1981-1982.
also, you can use this article about MMP-2 assay with ELISA and gelatine zymography. It can be usefull for understanding the correlation between this two assay methods
- Smith, L., A. S. Marenghi, and S. J. Capper. "Determination of species cross-reactivity using MMP-2 Activity Assay and correlation with ELISA and gelatin zymography." Functional genomics of model organisms (1999): 9.
If you need these two articles, please dont hesitate to contact me.
Sincerely
Dear Dr. Marcia Moss
The requested articles were sent to your email.
Best regards
Thank you Masood, your refered article seems good. Could you send them to me.
My email is rafiei1710#gmail.com
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