Alireza Rafiei

5 Questions 5 Answers 0 Followers

Questions related from Alireza Rafiei

What is the number of Porphyromonas gingivalis equivalent to 0.5 OD600 (MacFarland) standard?

Dear All, if OD600 = 0.5, is equal to how many Porphyromonas gingivalis cells ?

10 June 2018 4,612 2 View

Does anyone have an experience on gene expression from FNA (fine needle aspiration) - paraffin embedded liver tissue?

We used FNA paraffin-embedded hepatic tissues from our study population to evaluate some gene expression. But already we got week results in RNA extraction and also in RT-PCR. We extracted RNA by...

12 July 2017 1,996 3 View

How can I do haplotype analysis in a large case control study?

I want to know haplotype association with chronic diseases. Therefore, we have done a few case-control studies and pooled SNPs data in statistical software such as SPSS (Ver17). Is there any path...

04 April 2014 216 2 View

How can we present the absolute/relative values of real-time PCR of a target gene in comparison to a high positive calibrator gene?

I ran some experiments to quantitatively evaluate the expression of the products of a few genes in some cell lines. For calibration, I used the positive cell line which reported full expression of...

31 December 2013 6,710 2 View

Does anyone know of studies comparing the results of serum or urine matrix metalloproteinase-9 (MMP9) with the results of geletin zymograghy?

I'm measuring MMP9 in urine samples by using ELISA method. As the ELISA measures proenzyme and it's active form, to separate these forms usually uses geletin zymograghy. It would be appreciated if...

01 November 2013 9,060 6 View