Are cloud points able to identify tree species?

Hello Dears My goal is to classify dense forest tree species using drone images. Is it possible to classify dense cloud points? If yes, in what way and in what software? Thankful

15 February 2021 3,475 3 View

Do you have any information on modeling the diameter growth of conifers?

Hello dear friends Regarding modeling the growth diameter and cross section of conifers, I need a number of articles that have researched in this field. have you any recommendation? Thank you for...

20 January 2021 6,532 4 View

What is the classification of tree species by texture analysis technique on drone images?

Hi My goal is to distinguish forest tree species (in dense forests) with drone images. I did not get acceptable results using spectral features. Because the pictures were taken in the fall and the...

07 January 2021 7,494 3 View

How to classify a base object with drone image?

Hello beloveds. I am classifying dense deciduous trees with eCognition software. In fact, it is the separation of tree species with drone images. The image has a near infrared band, red, red...

29 December 2020 5,900 2 View

What is the best flight altitude for a drone in the forest?

My goal is to use the short-range remote sensing technique (drone) to study tree species in deciduous forests. Overlap and flight altitude play a key role in this. Do you have the right overlap...

02 December 2020 2,442 4 View

What are new trends in heat transfer and fluid mechanics?

Hello I'm currently searching for new trends related to heat transfer and fluid mechanics to select my M.Sc. thesis topic. can you please introduce me innovations and recent advancements in this area?

30 November 2020 9,316 13 View

Does anyone know what is an appropriate statistical analysis of drug release studies?

I am measuring the drug release of a few drug-eluting implants in dissolution medium in different time intervals. All the measurements are in triplicates. Would ANOVA or TTEST is an appropriate...

20 September 2020 8,183 5 View

When should I harvest the yeast cell growing in galactose medium to perform qPCR?

Hi, I engineered the yeast Saccharomyces cerevisiae to over-express some of its native genes, in addition to native copy of gene. For overexpression, I used strong modified GAL1 promoter ( that...

12 November 2018 496 3 View

Is there any enyzem/protein that can target a short DNA sequence (not promoter), and this complex can then behave as a regulator?

I am searching for a protein (or transcription factor) that can interact with a short DNA sequence (that is NOT promoter or some part of promoter for another gene) and this interaction results in...

18 April 2018 4,917 2 View

How to design DNA methylation primers?

Hi colleagues Will you please direct me how to design primers for DNA methylation and unmeythelation for control? plus i want you to inform me with details and what should i demand for the whole...

02 March 2018 6,690 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View