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Questions related from Gita Naseri
does someone know a paper where authors engineered the arabinose induction system of prokaryotic organisms in yeast Saccharomyces cerevisiae, like an IPTG induction system, for orthogonal gene...
04 November 2021 7,894 3 View
I am going to make a list of genes with unknown functions in Salmonella typhimurium strain LT2. To make a comprehensive list, I wondered if someone knew a publication or database that could help me.
01 November 2021 9,666 2 View
I am searching for software to design gRNA for cas12-mediated downregulation (CRISPRi) in Salmonella. Could someone suggest to me a software?
01 June 2021 637 1 View
I need to integrate a plasmid into the genome of Pichia pastoris. My plasmid encodes proteins URA3 and TRP1. Therefore I am searching for a P. pastoris strain that is not able to grow on SD-URA...
14 December 2020 9,285 0 View
I have a cell that expresses a luciferase protein upon adding substrate. Our luciferase protein has an excitation wavelength of 488 nm and therefore it is supposed to be able to excite a yellow...
04 December 2020 6,764 2 View
I have a plasmid that allows expression of a gene under the control of an artificial promoter. This promoter contains core part of CYC1 promoter (cyc1 minimal promoter) from Saccharomyces...
21 October 2020 6,268 3 View
I would like to measure AcGFP1 and DsRED intensities in cells that are producing beta-carotene using FACS (the cells are producing AcGFP1, DsRED, and beta-carotene). Because the cells are...
28 February 2019 3,260 3 View
Hi, I engineered the yeast Saccharomyces cerevisiae to over-express some of its native genes, in addition to native copy of gene. For overexpression, I used strong modified GAL1 promoter ( that...
13 November 2018 806 3 View
I am searching for a protein (or transcription factor) that can interact with a short DNA sequence (that is NOT promoter or some part of promoter for another gene) and this interaction results in...
19 April 2018 5,185 2 View
I have library of plasmids and I am looking for a highthroughput method to sequence whole library instead of sequencing one by one. Could someone suggest any method?
20 October 2016 7,208 5 View