Gita Naseri

7 Questions 6 Answers 0 Followers

Questions related from Gita Naseri

Does anyone know of a software to design gRNA for CRISPR/Cas12a-mediated downregulation in prokaryotic cell?

I am searching for software to design gRNA for cas12-mediated downregulation (CRISPRi) in Salmonella. Could someone suggest to me a software?

01 June 2021 575 1 View

Do you know anyone who has Pichia pastoris strain that is mutated for gene URA3 or gene TRP1?

I need to integrate a plasmid into the genome of Pichia pastoris. My plasmid encodes proteins URA3 and TRP1. Therefore I am searching for a P. pastoris strain that is not able to grow on SD-URA...

14 December 2020 9,203 0 View

Is that possible to measure luminescence output using FACS?

I have a cell that expresses a luciferase protein upon adding substrate. Our luciferase protein has an excitation wavelength of 488 nm and therefore it is supposed to be able to excite a yellow...

04 December 2020 6,702 2 View

Can I measure GFP and RFP intensity using FACS in cells expressing beta-carotene?

I would like to measure AcGFP1 and DsRED intensities in cells that are producing beta-carotene using FACS (the cells are producing AcGFP1, DsRED, and beta-carotene). Because the cells are...

28 February 2019 3,207 3 View

When should I harvest the yeast cell growing in galactose medium to perform qPCR?

Hi, I engineered the yeast Saccharomyces cerevisiae to over-express some of its native genes, in addition to native copy of gene. For overexpression, I used strong modified GAL1 promoter ( that...

13 November 2018 609 3 View

Is there any enyzem/protein that can target a short DNA sequence (not promoter), and this complex can then behave as a regulator?

I am searching for a protein (or transcription factor) that can interact with a short DNA sequence (that is NOT promoter or some part of promoter for another gene) and this interaction results in...

19 April 2018 5,039 2 View

How next generation sequencing can be implemented to sequence library of plasmids?

I have library of plasmids and I am looking for a highthroughput method to sequence whole library instead of sequencing one by one. Could someone suggest any method?

20 October 2016 7,148 5 View