Does anyone know of a proximal tubule specific marker of basolateral membranes?

More Brandon A Kemp's questions See All

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Why do we use Horse Serum for C2C12 cell differentitation?

I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA. What's the...

04 July 2024 4,732 1 View

Installing Climate Data Operators?

Hello, I am trying to run Cygwin terminal but I experience a problem of cyproj-13.dll not found as below. I even checked on Cygwin but it is not there. Kindly help.

11 June 2024 4,502 0 View

How I can differentiate between semi metals and metals?

I've seen many papers on GaAs with a weak density of states at the Fermi level, but more above and below when the conduction band descends and passes through the Fermi level until it crosses and...

09 May 2024 4,503 1 View

What is the best way to collect and preserve leaf and fruit samples for secondary metobiltes extraction?

The samples will be collected from the middle of Borneo rainforest so I am trying to find a way to keep the samples from deteriorating for around 2 weeks trip. The method should be simple and...

21 January 2024 2,184 1 View

What is the best way to resuspend a cell pellet?

I was having a discussion with a friend about the best way to resuspend a cell pellet, flicking the tube or pippeting up and down. I wanted to bring the discussion to this community and ask what...

21 December 2023 4,326 1 View

Colony PCR Not Working?

I have inserted a 300bp sequence of interest into the PTRV2 vector for use in VIGS and transformed that vector into E.coli, plated on LB Agar w/ 1% Kanamycin. There was growth on the plates, but...

31 October 2023 5,559 4 View

Why is my molecular ladder still curved?

I’m not sure why my PCR bands aren’t clean and rectangular. I am using a 1% gel (50 mL TAE buffer with 0.75 g agarose), and I am loading 1 uL of 6x loading dye with 5 uL of PCR sample. I run at...

09 October 2023 3,382 3 View

Thawing OCT embedded tissue in RNAlater?

As OCT creates many downstream issues in the RNA analysis pipeline, has anyone tried thawing OCT tissue (smallish pieces) in prechilled (-20C) RNALATER-ICE? Since OCT is water soluble shouldn't...

24 September 2023 3,436 1 View

It is needed for a supercell band structure unfolding? If yes, how I can do it in SIESTA?

I don't understand how I can do it

09 September 2023 3,265 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View