Does anyone know of a good substitute for the LightCycler FastStart DNA Master SYBR Green I kit (capillary)?

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Can a random-intercept cross lagged panel model (RI-CLPM) be indentified with just two measurement points?

I am currently planning out my masters' thesis design and will be working with a large data set with variables that have been meseasured at two time points. Intially I thought of just using a...

21 January 2021 9,384 4 View

Why fouled membrane has more negative zeta potential and lower IP?

I modified PES membranes with nanoparticles (nanosilver, graphene, halloysite). They were used in UF for WWTP effluent treatment. After filtration, all membranes exhibited more negative zeta...

26 December 2020 5,676 3 View

Will making a hydride acceptor more electrophilic speed up hydride transfer?

I have been searching the literature about this and I have not found an answer. If the atom that accepts a hydride is made more electrophilic, can this speed up the rate of hydride transfer?

29 July 2020 9,944 1 View

Can the energy displayed in Gaussian Calculation Summary be used to compare the stability between molecules?

I want to compare the stability of molecules that have the same number of atoms. I optimized these structures and calculated their vibrational frequencies. Gaussian09 gives a Gaussian Calculation...

18 July 2020 5,243 5 View

Could anyone suggest some articles and reliable sources about the (expensive) price of ruthenium and its complexes fabrication?

I'm writing and article about dye sensitized solar cells and I need a reliable source (articles, journals, books, reviews...) to base my statement (that ruthenium itself and ruthenium complexes...

07 February 2020 8,172 1 View

Can I use different rotation tecniques to compare extract factors in different years?

Dear researchers, I obtained a rotation matrix (varimax) in my factor analysis with correlation no higher than 0.20 for my 2010 data. However, for my 2014 data, with varimax rotation, I obtained...

20 January 2020 5,393 2 View

Do you know of any (ideally German) open source intelligence measures?

We are planning a study, in which we want to measure verbal, numerical, and spatial intelligence in university students. The study takes place in a German-speaking country and we had originally...

19 August 2019 175 4 View

Which temperature should I use in Molecular dynamics (GROMACS) for a protein found in endoplasmatic reticulum?

Hi everyone, I'm trying to perform a MD simulation for a protein found in ER. In mdp file should I set: ref-temp= 300 K or 310K? gen-temp should be the same than ref-temp or 300...

22 June 2019 2,083 6 View

No title

I'm developing a MethyLight assay to verify a specific CpG site in a promoter region of my interest gene in rats. Unfortunately, I don't have a lot of choices to design the probe and primers,...

20 June 2019 1,004 3 View

How to compare two RMSD plot (frame vs frame) from gromacs?

I have two proteins. One is wild-type and the other is a mutant. The RMSD plot (nm vs time) showed me mutant is far more unstable. But, when a plot the RMSD frame vs frame graphic it seems mutant...

28 May 2019 7,260 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Calculating volume of DNA required from DNA concentration?

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26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Steve Hawkins

Most fast mixes can be used, however if you are using glass capillaries, this does requires the addition of BSA to the qPCR reaction at a final concentration of 250ng/mL in order to prevent the DNA polymerase and template from binding to the glass.

Hassan Nima

agree with @ Steve Hawkins

regards

Khalid Al-Salhie

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