Does anyone know if there are ATP synthase subunit beta isoforms (ATP5B)?
This is the mitochondrial ATP synthase.
Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...
22 July 2024 2,257 0 View
Best method and number of embryos for extracting RNA from pig morulae?
21 July 2024 4,398 1 View
eliminoar articulos que son de tu autoria
05 July 2024 2,107 1 View
It is required to make a precursor gel for a SAPO-11 Zeolite, the compounds are the following: * As a source of Al: Aluminum Isopropoxide (98%) Formula: C9 H21 O3 Al PM: 204.24 g/mol Density:...
01 July 2024 617 3 View
Do you think the use of virtual reality with dyslexia students is good?
23 June 2024 8,437 2 View
Hola, Soy una estudiante de primer año de la carrera de Antropología en Chile, siendo parte del alumnado de la Universidad de Concepción. Se me ha asignado junto con mi grupo el trabajo de...
16 June 2024 2,715 4 View
Hello, Can somebody please give me some reference to a paper or book where it is explained how to condensate: * from a Q8 serendipity FE to a Q4 FE with drilling DOFs (16 DOFs to 12 DOFs). *...
05 June 2024 936 1 View
Hi everyone, I'm having trouble with my hydroponic cultures for Arabidopsis thaliana, since lately I've started having algae contamination on them. I have tried using several sterilization...
21 May 2024 4,840 1 View
Hello, I would like to inquire about how the expansion of the cumulus can be measured during or after IVM. Thank you.
16 May 2024 9,330 2 View
Hello community! What is for you the best combination of media for IVM, IVF and CIV in mice? In my experience we usually use media made in our laboratory but I have the opportunity to purchase...
02 May 2024 2,548 0 View
Hello everyone We are building an atomic structure model against a cryo-EM map of AAA+ ATPase. Considering that AAA+ polymerizes when ATP is added, we collected data for AAA+ mixed with ATP. Our...
04 April 2024 4,640 2 View
I am trouble shooting a malachite green ATPase assay using a kit from Sigma (MAK307) for a cytosolic protein that converts ATP --> ADP + Pi and am wondering what cheap + commercially available...
30 March 2024 9,659 1 View
Hello! I'm plannning to investigate the relationship between mitochondrial mass/function and epithelial homeostasis/differentiation. Are there any activators(drug) of mitochondria interms of...
27 March 2024 8,273 0 View
I need to order an ATP Assay Kit for my work, and I found this kit on the BT Lab website. Has anyone tried the kits from this site? Because this is the first time I will order from them.
27 February 2024 6,940 1 View
Hello, I performed the ADP-Glo kinase assay. In that case, samples(active protein+substrate+ATP+Kinase buffer+w/wo test compound mixture) were incubated for 45 minutes in 30℃. In the next...
27 December 2023 8,915 2 View
I am working with HepG2 cells to observe effects of Simvastatin treatment on the cells. We are aiming to identify a dose response associated with levels of ATP, metabolic activity, and CoQ10...
29 November 2023 6,062 0 View
I am currently trying to develop an ATP concentration measurement protocol for my human cultured cells. However, I have found little to no detailed protocol for ATP extraction using TCA...
27 November 2023 7,980 1 View
Hi dear All. I'd like to know simplest protocol to detect ATP binding assay (ATP binding with protein). I read a couple of research about this but I want to know common methods. Thanks for your...
19 November 2023 7,412 0 View
process of human obtaining energy in cellular respiration
28 October 2023 7,303 1 View
I’m having issues with the stability of ATP. My lab has purchased multiple brands of ATP (Sigma # A6419; SantaCruz #SC-202040), each of which seem to come hydrolyzed. My lab uses the Malachite...
18 October 2023 3,648 0 View