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Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

Must a control group to be in the same semester that a experimental group for a study in education?

Hi everyone, I'm researching about mobile learning in higher education. Due to the small number of the students in the semesters, I cannot have a experimental and control group together. I'm...

22 February 2021 3,653 11 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View

What data analysis is applicable if my study will employ a Likert scale to establish association between certain factors and an ordinal variable?

My study aims to find what factors that lead to nurse stress are most significantly associated with nurse absenteeism. So the questionnaire will ask about these factors through a Likert scale...

17 January 2021 7,388 8 View

Proper RP-HPLC workup following peptide synthesis?

Hello, I've recently synthesized a short (17aa) peptide via manual SPPS. I was instructed to submit a small pipette tip's worth of material onto a capillary column to obtain a rough estimate of...

12 January 2021 9,432 3 View

SPPS: peptide failure to elongate?

SPPS of a 16 aa peptide was performed on a 0.15 mmol scale. Resin gained a minimal amount of weight: starting weight of 224 mg and a final weight of 280.I believe my peptide failed to elongate but...

21 December 2020 9,428 11 View

Does freezing treated cell pellet at -80C damages them?

Hello, I hope you are all doing well. I have a question regarding my treated cells group. I plan to do western blot and qRT-PCR for my treated cells. However, to save time, I would like to...

06 December 2020 1,390 5 View

RNA-Seq for the analysis of ribosomal RNA (rRNA) processing sites?

Greetings! I want to study ribosomal RNA expression and potential processing sites in bacteria. To such effect, I want to perform an RNA-Seq experiment in order to analyze potential rRNA...

02 December 2020 1,310 2 View

How should I dispose of silver staining waste (SDS-PAGE)?

I know I cannot pour silver staining waste down the sink, but how should I dispose of it? Thank you in advance!

18 November 2020 1,745 3 View

Problem executing MrBayes. Help?

Hi, I'm trying to run MrBayes 3.2.7 with a matrix of 37 species and 20561 nucleotides. I consistently receive the following error: MrBayes > Execute...

18 November 2020 3,867 3 View

How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

15 February 2021 3,746 4 View

What is the time required for an enzyme or an protein molecule to consume an ATP molecule during a reaction?

In what time do a protein body consumes an ATP molecule for an active process to happen?

02 October 2020 8,135 4 View

What are the effects of phosphorus deficiency on dehydrogenase activity in soil bacteria and fungi?

I suspect that under phosphorus deficiency the electron transport chain in the plasmamembrane and mitochondria of soil bacteria and AM fungi, respectively, is disrupted as a result of lower ATP...

30 August 2020 4,659 3 View

Please recommend biological investigation of newly identified anticancer compounds that specifically inhibit Src kinase?

We aim to publish ta high impact journals (If 6+). Compounds showed incredible activities on cancer cell line (submicromolar on several types). I need your recommendation on how to determine...

24 May 2020 1,230 3 View

Monitoring ATP binding to proteins: TNP-ATP or MANT-ATP?

Hi all, I'm interested on monitoring ATP binding to a protein of interest using fluorescent ATP analogs, such as TNP or MANT-ATP. Do you have any experience with them? Which one is the best...

03 May 2020 3,993 5 View

ATP content measurement?

Hello, I am looking for a clear protocol for ATP content assay. I am not looking for a kit because it is expensive. I am looking for a fluorogenic or a colorimetric substrate that helps me to...

24 March 2020 2,286 3 View

ATP evoked calcium response in skin fibroblasts: no response?

23 March 2020 4,854 2 View

Does POM1 cause precipitation with ATP?

Hi, I've been doing multiple experiments to measure POM1 inhibition of ATP breakdown by CD39. I measure the amount of breakdown using either the malachite green assay or the ATP luciferase...

17 February 2020 5,340 1 View

Is ATP the best energy providing molecule? can there be something better?

ATP energy-storing molecule as is no doubt an efficient! as not only it is used as energy storage and provider in all forms of life and has probably been around for billions of years. Even in the...

08 February 2020 9,015 6 View

How to measure Km for ATP with Kinase Glo Plus?

Dear colleagues, Is there anyone who worked with Kinase Glo Plus reagent? I have to measure Km for ATP for ATP-hydrolyzing enzyme in the range from 0.3 to 100 mcM. Is it possible to do it...

11 December 2019 9,661 2 View