How to start with STZ induced diabetes in mice?

I would like to start a project of inflammatory bowel disease in diabetic mice (diabetes induced by STZ) and I hoped you could help me with the methodology. I know the theory form literature but...

09 October 2013 3,808 2 View

Proper marker for neutrophiles in colon tissue?

I am planning to do immunohistochem with markers for neutrophiles (activated and not activated). Maybe you have some suggestions what marker should I use. I was thinking about CD6. I read it...

06 July 2013 6,622 4 View

Primary smooth muscle cells culture contaminated with fibroblasts.

I am isolating smooth muscle cells form abdominal aortic aneurysm tissue. In brief I cut obtained tissue into small pieces and digest it overnight. The cells grow very well in M199 medium with 10%...

14 February 2013 3,285 17 View

Recommendations for ELISA plate reader.

I am going to buy an ELISA plate reader. Can you advise me on something reliable and reasonably priced?

01 February 2013 175 3 View

Primary smooth muscle cells culture contaminated with fibroblasts.

I am isolating smooth muscle cells form abdominal aortic aneurysm tissue. In brief I cut obtained tissue into small pieces and digest it overnight. The cells grow very well in M199 medium with 10%...

01 February 2013 7,582 18 View

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03 March 2021 5,360 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View