Recommendations for ELISA plate reader.

More Aleksandra Piechota-Polanczyk's questions See All

Does anyone know if RV1B and PIV3 viruses replicate in polymorphonuclear leucocytes?

Hi everyone! I am planning to conduct some experiments infecting blood PBMC with RV1B and PIV3 viruses. Do you know if blood PBMCs are infected by RV1B and PIV3? Can PIV3 and RV1B replicate in...

10 November 2014 793 0 View

How to start with STZ induced diabetes in mice?

I would like to start a project of inflammatory bowel disease in diabetic mice (diabetes induced by STZ) and I hoped you could help me with the methodology. I know the theory form literature but...

09 October 2013 3,808 2 View

Proper marker for neutrophiles in colon tissue?

I am planning to do immunohistochem with markers for neutrophiles (activated and not activated). Maybe you have some suggestions what marker should I use. I was thinking about CD6. I read it...

06 July 2013 6,622 4 View

Primary smooth muscle cells culture contaminated with fibroblasts.

I am isolating smooth muscle cells form abdominal aortic aneurysm tissue. In brief I cut obtained tissue into small pieces and digest it overnight. The cells grow very well in M199 medium with 10%...

14 February 2013 3,285 17 View

Primary smooth muscle cells culture contaminated with fibroblasts.

I am isolating smooth muscle cells form abdominal aortic aneurysm tissue. In brief I cut obtained tissue into small pieces and digest it overnight. The cells grow very well in M199 medium with 10%...

01 February 2013 7,582 18 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Nadine A. Gund

Dear Aleksandra, we have a Tecan Sunrise plate reader (http://www.tecan.com/platform/apps/product/index.asp?MenuID=1260&ID=682&Menu=1&Item=21.2.10.4). The support is quite well.

I don't know the price (maybe it's different in different countries?) but maybe I can tell you the price tomorrow.

Cheers, Nadine

Rasool Jafari

Anthos folido would be a good choice.

Stephan T. Kiessig

8 channel, dual wavelength detection (one for you substrate, the second one for optical errors), wavelength according to your substartes (TMB in HRP = 492 nm, ...)

Connection to PC, the calculation of calibration curves and samples ist much mor comfortable using external PC, sometimes shakers are includes (it's can be helpful but it's not a prerequisite)