I am planning to do immunohistochem with markers for neutrophiles (activated and not activated). Maybe you have some suggestions what marker should I use. I was thinking about CD6. I read it indicates activated neutrophiles.
Hi,
The largely used marker for neutrophil mapping is the Gr-1 otherwise I rather use the Ly6-G antigen.
Gr-1 antidodies recognize either Ly6-G alone or the couple Ly6G-Ly6-C and are not 100% specific for neutrophils (it cross reacts with a subtype of myeloid cells).
We are mapping neutrophil in mouse lungs using Ly6-G antidoby (clone 1A8) from Biolegend.
For activation status, activated neutorphils are CD11b pos.
Good luck
Alexis
None of the markers are specific for Neutrophil. Still, in combination of Ly6G and CD11b, you can give a try .
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