I'm looking for a better viruses response method (of course, without vírus).
It would depend a lot on: (1) the cell types (cell models) you want to investigate or characterize; (2) the phenomena you want to explore or explain based on your hypotheses (e.g. a hypothesis on whether xyz virus infection leads to ferroptosis; or whether autophagy aids or checks viral infections, or whether an uncharacterized compound can activate inflammasomes).
When you have some pathways in mind (that it may be linked to some cellular processes of interest), then it would be easier to make choices and narrow down the scope of tests needed.
The biotechn company INVIVOGEN sells a host of high-quality products for immunological investigations, such as immunostimulants (ligands), enzyme inhibitors, reporter cell lines, and inflammation inducers.
You can check out their catalogues.
https://www.invivogen.com/ligands
For example, dsRNS mimetics (.e.g. poly(I:C)), TLR3 ligands, etc., may be some of items that you may want to look into for setting up a non-viral model that captures aspects of virally induced inflammation.
The work-flow:
(1) decide the cellular process you want to focus on; (make sure it's not well reported in PubMed...)
(2) pick a key cell type involved in the process (check literature or based on experimental evidence)
(3) pick a pathway (receptors, transducers, transcription factors, gene expression machinery, etc.) that supposedly mediates the process (either theoretically or based on intuition)
(4) pick the right pharmacological tools: stimulants, inhibitors, antibodies, primers
(5) have a reliable and reproducible assay for your hypotheses.
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