Calculate pH after addition of base?

04 February 2021 2,232 4 View

Western blot with very acidic proteins, any suggestions?

I am trying to do a Western Blot to detect acidic proteins (~30kDa) that have a PI of ~3.5, due to the presence of Asp and Glu repeats. The proteins are about 80% pure. After I ran them on...

24 January 2021 7,226 3 View

How does one create IFORM contours?

Hello: Can anyone assist with plotting IFORM contours. I have been trying to plot contours using Hs and Tp as variables. The marginal distribution is a GPD-Kernel density and the conditional...

12 December 2020 4,355 3 View

How can I transfer heat between groups in Gromacs?

Is it possible to transfer heat from one group to another using simulated aneeling technique in the Gromacs software? I would like to transfer heat from a gold nanoparticle (group 1 from 310 to...

20 November 2020 9,062 3 View

Would ER NiCrMo-3 be the best option for weld overlay of carbon and low alloy steels?

Alloy 625 is the most popular alloy for weld overlay. Without considering the price factor, would NiCrMo-3 be the best technical option for weld overlay of API 5L and low alloy steels, in general,...

24 August 2020 2,478 4 View

Dose-response on non-Michaelian response enzymes, how?

Hello For typical dose-response assays, our lab usually uses steady state intervals for defining the difference between control and tested compound. For those assays, we typically use the angular...

23 July 2020 8,662 2 View

What is the best way to graph an SVM classification in R or Python?

I'm trying to find a way to graph a classification made by the support vector machine algorithm. Something like a PCA or LDA chart would be excellent. Any help will be welcome.

26 April 2020 4,588 1 View

Gene therapy with transgenics viruses Covid-19?

Viral load and symptomatic picture Is there any relationship between the viral load of exposure and contamination, in number of viruses and contamination processes, and the symptomatic picture in...

08 April 2020 6,349 2 View

Is it allowed to publish part of a journal paper(under review) as a conference paper?

Is it allowed to publish part of a journal paper under review as a conference paper? I have submitted a full research paper to a journal. I'm waiting for the review answer and I want to submit...

01 April 2020 430 6 View

Do KG-1a human macrophages need to be activated in some way?

Hello everyone. I was wondering if you can help me clarify this question. I am seeking to perform infection assays with bacteria, using this human cell line. Research articles regarding infection...

24 March 2020 9,884 3 View

How was your feeling, when your first research paper was published ?

Well, writing a research manuscript is an intimidating process for many new writers and one of the stumbling blocks is the beginning of the process and creating the first draft. Please share your...

29 December 2020 5,180 15 View

What medical specialties and sub-specialties should be included in a history of medicine bibliography?

I'm in the final stages of compiling a history of medicine bibliography. It will be my second reference work to be published by Jenny Stanford Publishing (my first was "The Nanotechnology...

06 December 2020 3,825 3 View

Do you think, citing a "preprint" as reference is logical in your future article?

Now a days many researchers are depositing their research paper draft as preprint in various web-based option, those are not peer reviewed yet or may submitted already or will be submitted in a...

29 November 2020 6,849 2 View

How to approach native speaker to be a co-author and how to build trust?

For non-natives, a manuscript is often rejected based on the reason that idea is not presented clearly. Please ask a native colleague to proofread it. There is no offense to say that I am...

26 November 2020 7,495 12 View

Which platform will you recommend for plagiarism checking at an affordable cost?

Often, we have to check plagiarism for our research papers. However, there is no free-reliable plagiarism software. Can you suggest any plagiarism checking platform at an affordable cost? Please...

19 November 2020 3,883 8 View

Analytical Assessment for L2 Writing?

I am using "essay assessment" as a third data type in my research. There are 80 essays: first draft and final draft. a. In order to get a general picture of students' level of writing, should I...

08 October 2020 201 2 View

Can you suggest an academic platform (in addition to RG and Academia) where PhD students can share drafts of their papers for feedback?

I am looking for a platform where PhD students would feel comfortable to share their papers at a very early stage of research and could receive a helpful feedback.

05 August 2020 4,713 3 View

What is the difference between systematic review and meta analysis ? How to do meta-analysis ?

Nowadays, meta analysis is being widely preferably used for review paper writing. Is this meta analysis having edge over systematic review articles? Is there any specific software or methodology...

07 July 2020 3,172 14 View

If I Have one Group of Participants, is There a Statistical Analysis for this?

5 August 2020: Looking for assistance on a review of the calculation of my work on Kendall's W and Tau. This method made more logical sense in that its heavily used for survey responses. I also...

27 June 2020 5,983 4 View

Frustration from failing to publish papers. Can anyone suggest what to do?

Consecutive "Rejections" of submissions from journals may lead researchers to mental distress. How to overcome that?

21 June 2020 3,020 73 View