Do you know any voice dataset of twins?

I'm working on research on speaker recognition performance on twins. Would be grateful for any links to datasets that contain voices of twins!

08 December 2020 745 1 View

EMG control in ALS patients. Any experiences?

Dear everyone! I work on EMG control and EMG interfaces for bionic (myoelectric) extremity reconstruction. I have a relative with ALS and was wondering if anyone has experience in controlling a...

22 August 2020 8,013 2 View

How to get data from social networks for text mining?

I am interested in a software tool for extracting data from social networks based on geographic characteristics. Purpose - analysis to obtain data on the mood of the population.

17 August 2020 7,326 3 View

Carbon Nanotubes Dispersion: your recipe?

Hello to everyone! My new project is related to adding organic dispersion on MWCNT (NC7000 if matters) into paraffine. Now I use toluene and ultrasound treatment (high intensity) to make one,...

22 March 2020 6,118 4 View

Could you promted me publications about this type of model?

I have created the model of population dispersion to use it for Arianta arbustorum land snail populations investigating. You can see this model here...

07 March 2019 5,105 2 View

Synergy scores: HSA and Bliss positive, but Loewe is negative. How to intepret?

Hi everyone! Recently I was doing a lot screening of drug pairs in 5 osteosarcoma cell lines using this amazing website https://synergyfinder.fimm.fi. I am puzzled why a lot of combinations have...

08 May 2018 8,856 3 View

How to select genes for MLST?

28 December 2016 1,512 2 View

Western Blot - How much dry milk in the antibody solution?

04 October 2016 6,540 12 View

Has anybody an idea how to evaluate confluency?

I'am looking for a method to examine confluency of cells in pictures taken by a microscope-camera application (sample picture is attached). It would be very nice if you could help me with a method...

04 August 2016 6,705 13 View

Confluency determination with human granulosa cells?

Dear community, i'm looking for a possibility to determine the confluency of granulosa cells in a dish. We stimulated cells with Pam3Cys-Ser-(Lys)4 (a TLR2 agonist) and observed them under the...

25 July 2016 6,636 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View