I am differentiating monocytes into macrophages from PBMCs, and every time I have encountered these bacillus-shaped elements when I looked them in the microscope at 40x. They are not typical bacteria because I have taken the supernatant from some wells and incubated it with LB medium, but nothing grew. Additionally, the medium did not turn yellow. I have seen it in different cultures in which I used different reagents and donors. Cells do not look atypical.
I left you a video of the culture
Could anybody help me to identify this elements? Are they normal? Some advice?
Thank you very much!
Hi Daniela,
We did see something similar at some point in our primary equine BMDM... they looked a lot smaller though. We sent it to culture in a variety of media conditions and we consistently got Paenibacillus Etheri which was ultimately identified to come from the culture plates.... addition of gentamycin to the culture media was enough to control it, but we discarded that batch of plates though.
Thank you very much B. C. Menarim !
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