10 May 2024 1 8K Report

Hi - I am looking for advice on protocol for subculturing and feeding RD-ES line. THey seem to grow semi-adhered and not sure if I should treat them as cells in suspension or adhered. Thank you!

More Daniela Cox Beltran's questions See All
Trypan Blue overestimates the viability of cryopreserved PBMC?

Hello! Have you ever observed that when measuring the viability of freshly thawed PBMC with trypan blue, the value is higher than with Annexin/PI staining or some other staining that takes into...

10 July 2024 640 1 View

How to do a systematic review?

Hello, I am working on a systematic review. I have my topic, and I am searching Scopus and Web of Science, but I want to search Google Scholar to compare the results. Does anyone have any advice...

10 June 2024 9,602 0 View

Maximum passage for Ewing sarcoma cell lines?

I was hoping anyone could provide any insight into using ATCC's "RD-ES" and "A-673" cell lines. How many passages do you do before you notice any senescence or genetic abnormalities

20 May 2024 596 4 View

Research about impact of brave and persistence?

I want to read about studies or experiments who proved that being brave and persistent are suceeding factors. Are there any new aspects or findings e.g. from neuroscience?

12 May 2024 4,614 3 View

I am considering conducting a mark-recapture study on crickets. Can anyone recommend non-toxic marker substances?

I am considering conducting a mark-recapture study on a closed population of wild crickets. Can anyone recommend non-toxic marker substances?

05 May 2024 5,942 1 View

Do you suggest any web based or app that could be used to manage bookings of lab related equipment?

We would like to manage in an efficient way our newly conceived flow cytometry and and would like to find a good way to manage our bookings Please Help

21 April 2024 845 1 View

Hello everyone! Would you please help me identifying this marine polychaete ?

It was extracted from a sediment sample in the subtidal zone

19 April 2024 6,725 4 View

How to perform the embedding and cutting of 0,4 um transwell Pet inserts to visualize polarized cell layer for histology?

i seeded CACO2 on 0,4 um PET transwell insert, i want to see my cell layer in transversal view in order to monitor the polarization and formation of brush border. To do this, i fixed with 4% PFA...

16 April 2024 6,517 1 View

Is this a good titile?

"Architectural Impressions: Exploring the Lifelong Influence of Childhood Environments on Memory and Identity"

21 March 2024 6,448 1 View

How can I induce Netosis cell death in HL60 differentiated with DMSO?

Months ago i was able to induce NETosis in Hl60 differentiated cells using PMA while now with a different PMA solution I can't anymore. I followed the condition reported in literature of 100nM PMA...

18 March 2024 2,925 0 View

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