How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

How can I model a prediction of a dependent variable using BOTH a predictor at time 1, time 2 AND the change (time 2-1) of this predictor?

I want to model the following: DV: Y at time 3 IVs: X at time 1, X at time 2, change of X from time 1 to time 2 Which is the most appropriate way (preferably in SPSS). I am afraid I cannot use...

10 December 2020 264 3 View

Can you recommend me tree databases?

Hi. I have slight experience in searching phylogenetic trees, normaly I use TreeBASE or Dryad but some papers that I found doesn't have the resulting trees in these databases or there are genetic...

26 October 2020 582 3 View

Could ANOVAs be used as a substitute for regression models for mediation analysis?

Hello all, I hope you can help me with these questions. I am developing a mediation analysis using the mediation package in R. I need to analyze the influence of perceived learning (Mediating...

18 August 2020 7,521 9 View

How do you use factor analysis on categorical variables?

Hi, I'm trying to identify how similar different individuals are in their body traits. Normally, I would use a PCA or DFA, depending on my question. However, I have only categorical variables...

20 July 2020 4,171 1 View

What would be the fate of ecotoxicology in the South American studies?

I'm currently working on ecotoxicology and I'd like to know what my colleagues from the same area think, especially in South America. Too much of microplastics in my opinion, but few studies...

18 May 2020 1,064 2 View

Do plant extracts contain essential oil compounds?

So I understand that essential oils are formed by steam distillation, and contain volatile, usually aromatic, compounds. Extracts are formed by immersion of plant matter in a solvent, and are...

12 May 2020 2,119 5 View

(epidemiology) how does one measure the RATE of infectivity?

I am not an epidemiologist, nor am I studying epidemiology, this is a question born of sheer curiosity due to current events. I understand (more or less) the principle behind the basic...

19 March 2020 9,458 3 View

How can I clean insect exoskeleton?

I want to remove whole soft tissues and maintain intact the exoeskeleton

10 March 2020 4,345 6 View

How would you evaluate "Interpersonal Trust" for people who are sharing a 4-seat vehicle with people they do not know?

Hi. I am conducting an experiment and I would like to understand if people would be willing to share a 4-seat vehicle with people they do not know. I have thought that the personal tendency of...

10 December 2019 7,989 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View