I want to stitch two different proteins in one sequence so that blast can read it thoroughly. Any one of you in this community has any experience or idea about this?
31 December 2014 5,401 3 View
Indeed you have answered in a very beautiful way and provide sufficient data but my question is that what could be the possible explanation, i did not find any good in your discussion section.
11 December 2014 1,691 0 View
I want to stitch two different proteins. My next step is to generate my data using HHblits server. By putting both the proteins in one sequence, HHblits will only result in one part and will...
10 November 2013 2,537 4 View
DNA modeling
11 December 2012 398 5 View
Write your research proposal, following some easy steps
03 April 2012 6,734 2 View
Six steps to win international scholarships
02 March 2012 4,133 0 View
http://expertscolumn.com/content/silver-staining-protocol-stain-sds-page Protocol of silver staining of SDS-PAGE gels, I used it personally and it worked very well. Silver staining is much more...
02 March 2012 6,729 0 View
protocol for silver staining SDS-PAGE.
02 March 2012 4,569 0 View
Six key steps to win international PhD scholarships
02 March 2012 7,602 0 View
Here is a silver staining protocol for your SDS-PAGE. Cheers,
02 March 2012 6,222 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...
07 February 2021 3,880 9 View
Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...
04 February 2021 4,482 4 View
Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...
02 February 2021 10,047 3 View
01 February 2021 1,825 1 View
I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...
31 January 2021 4,285 3 View
Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...
27 January 2021 8,702 3 View