I want to stitch two different proteins in one sequence so that blast can read it thoroughly. Any one of you in this community has any experience or idea about this?
Hello Everyone, I want to use NAMD to simulate transemembrane protein with copper as a ligand. Can anyone guide me how to fix the following issues; 1. Do I need to dock copper to my protein first?...
31 December 2014 5,517 3 View
Indeed you have answered in a very beautiful way and provide sufficient data but my question is that what could be the possible explanation, i did not find any good in your discussion section.
11 December 2014 1,801 0 View
I want to stitch two different proteins. My next step is to generate my data using HHblits server. By putting both the proteins in one sequence, HHblits will only result in one part and will...
10 November 2013 2,654 4 View
DNA modeling
11 December 2012 498 5 View
Write your research proposal, following some easy steps
03 April 2012 6,844 2 View
http://expertscolumn.com/content/silver-staining-protocol-stain-sds-page Protocol of silver staining of SDS-PAGE gels, I used it personally and it worked very well. Silver staining is much more...
02 March 2012 6,830 0 View
Six steps to win international scholarships
02 March 2012 4,240 0 View
Six key steps to win international PhD scholarships
02 March 2012 7,785 0 View
protocol for silver staining SDS-PAGE.
02 March 2012 4,675 0 View
Here is a silver staining protocol for your SDS-PAGE. Cheers,
02 March 2012 6,338 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.
24 June 2024 5,044 1 View
Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...
24 June 2024 8,622 6 View
Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...
12 June 2024 9,616 8 View
Hi everyone, I've been having difficulty in purifying a protein. It is a beta barrel, integral membrane protein. My protein is expressed and purified recombinantly. My target protein is running...
30 May 2024 7,604 3 View