How can one simulate protein with a Copper ligand in NAMD?

31 December 2014 5,401 3 View

How this paper can be discussed, as almost every paper is agreed on coupling mechanism from structure labs and NMR labs?

Indeed you have answered in a very beautiful way and provide sufficient data but my question is that what could be the possible explanation, i did not find any good in your discussion section.

11 December 2014 1,691 0 View

Can anyone advise on protein stitching?

I want to stitch two different proteins. My next step is to generate my data using HHblits server. By putting both the proteins in one sequence, HHblits will only result in one part and will...

10 November 2013 2,537 4 View

Does anyone know about protein sequence stitching?

I want to stitch two different proteins in one sequence so that blast can read it thoroughly. Any one of you in this community has any experience or idea about this?

10 November 2013 911 0 View

Can any one recomend me a software construct a desired DNA strand, please do not recommend me 3D Dart..

DNA modeling

11 December 2012 398 5 View

Write your research proposal, following some easy steps

Write your research proposal, following some easy steps

03 April 2012 6,734 2 View

Six steps to win international scholarships

Six steps to win international scholarships

02 March 2012 4,133 0 View

protocol for silver staining SDS-PAGE.

protocol for silver staining SDS-PAGE.

02 March 2012 4,569 0 View

Six key steps to win international PhD scholarships

Six key steps to win international PhD scholarships

02 March 2012 7,602 0 View

Here is a silver staining protocol for your SDS-PAGE. Cheers,

Here is a silver staining protocol for your SDS-PAGE. Cheers,

02 March 2012 6,222 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View