Six key steps to win international PhD scholarships

03 March 2012 0 8K Report

http://expertscolumn.com/content/tricks-and-guidelines-win-international-scholarships

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Science method

More Ashfaq Ahmad's questions See All

How can one simulate protein with a Copper ligand in NAMD?

Hello Everyone, I want to use NAMD to simulate transemembrane protein with copper as a ligand. Can anyone guide me how to fix the following issues; 1. Do I need to dock copper to my protein first?...

31 December 2014 5,517 3 View

How this paper can be discussed, as almost every paper is agreed on coupling mechanism from structure labs and NMR labs?

Indeed you have answered in a very beautiful way and provide sufficient data but my question is that what could be the possible explanation, i did not find any good in your discussion section.

11 December 2014 1,801 0 View

Can anyone advise on protein stitching?

I want to stitch two different proteins. My next step is to generate my data using HHblits server. By putting both the proteins in one sequence, HHblits will only result in one part and will...

10 November 2013 2,654 4 View

Does anyone know about protein sequence stitching?

I want to stitch two different proteins in one sequence so that blast can read it thoroughly. Any one of you in this community has any experience or idea about this?

10 November 2013 1,022 0 View

Can any one recomend me a software construct a desired DNA strand, please do not recommend me 3D Dart..

DNA modeling

11 December 2012 498 5 View

Write your research proposal, following some easy steps

03 April 2012 6,844 2 View

http://expertscolumn.com/content/silver-staining-protocol-stain-sds-page Protocol of silver staining of SDS-PAGE gels, I used it personally and it wo...

http://expertscolumn.com/content/silver-staining-protocol-stain-sds-page Protocol of silver staining of SDS-PAGE gels, I used it personally and it worked very well. Silver staining is much more...

02 March 2012 6,830 0 View

Six steps to win international scholarships

02 March 2012 4,240 0 View

protocol for silver staining SDS-PAGE.

02 March 2012 4,675 0 View

Here is a silver staining protocol for your SDS-PAGE. Cheers,

02 March 2012 6,338 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

31 July 2024 8,415 1 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View