Does anyone know about how long cardiomyocytes could wait in the incubator after isolation?

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Has anyone faced low transfection efficiency of SK-BR-3 and BT-474 cell with Lipofectamine 3000?

Hello. I've been trying to transfect plasmid DNA to my breast cancer cell lines (BT-474 and SK-BR-3) with Thermo Fisher's Lipofectamine 3000 reagent. According to the thermo fisher, those cells...

24 March 2024 9,489 5 View

Why are there so many deviations in my absorbance values in the MTT assay?

In the MTT assay, I read the absorbance values at 570 nm. However, sometimes there are so many deviations in the absorbance values. Hence, I have to take out some (up to 4 values) deviated values...

13 November 2023 4,928 0 View

Betwenness Centrality in Structural Connectome?

Hello everyone! I extracted betweenness centrality values of more than 250 data by using number of streamline weighted connectivity matrices. In order to calculate the betweenness centrality, I...

18 June 2023 4,839 3 View

Why can't I print tissue scaffolds properly?

Hello, I've been working with a 3D SLA printer to produce a bone tissue scaffold and trying different parameters, both the ceramic slurry and the 3D printer. However, I haven't achieved successful...

07 February 2023 3,169 6 View

How can I dissolve glyphosate?

I try to dissolve glyphosate in water with heat and without heat. I read an article that they mentioned using isopropanol or ethanol to dissolve glyphosate, but they did not explain the...

02 November 2022 5,294 3 View

How can use machine learning with the family data?

Hello everyone, I am planning to design a model that can predict individuals with high or low psychosis-proneness by looking at the brain connectivity measures in 114 regions, age, gender, IQ,...

12 September 2022 5,046 1 View

I have values from -4.5 to 10 in my database, and it is not normally distributed. Is there a valid way to transform the data into positive values?

I have one environmental risk score for psychosis (ERS) value for each participant in my database. The scores vary from -4.5 to 10. And the data is not normally distributed. I would like to ask...

29 July 2022 9,980 3 View

SacB counterselection troubleshooting?

Hi! I'm trying to create a deletion mutant using pEX18 Gm. I amplified my gene, cloned into pEX18 and with electroporation (1600v), my plasmid vector integrated into P.aeruginosa genome. But now...

12 March 2022 9,766 3 View

Specific gene region scanning?

Hi, I have the total genome sequence of an organism. I want to scan for the presence of a special gene from this sequence. How is it done, with which program should I do it? I would be very...

26 November 2021 6,206 3 View

How to extract FA and MD values for each participant if new data are added every week?

Hello Everyone, I have overall 150 data, and my professor asked me to extract each participant's FA and MD values. I know how to get mean FA and MD by using TBSS in FSL. However, since we are...

14 July 2021 1,759 4 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

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06 August 2024 641 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Yaroslav Tsytsyura

Hi! Ask this guy: https://www.researchgate.net/profile/Martin_Kahms/

He's prepped the carsiomyocytes from newborn mice, I know that they were kept in culture on coated coverslips for ~2 weeks, still beating (of course not longer looking like myocytes anymore but anyway)

Seda nur Topkaya

Thank you very much..