Seda nur Topkaya

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Questions related from Seda nur Topkaya

Is there anyone who works with Bacteriorhodopsin?

I have an urgent dissolution issue. I have a stock solution of br that I bought from Sigma. I checked the papers regarding the solution buffer. Is there any recommendation?

25 September 2014 3,663 4 View

What is the pH effect on UV spectra?

Could you please give me some information about the pH effect on the UV spectra? Is there a common tendency for pH changes or does it depend on the molecule which it is measured? Thanks in advance.

08 September 2014 7,192 15 View

From particles number to concentration - can anyone help?

Hi everybody I have 2.10 11 particles/ml gold nanorod solution. How I can calculate the concentration? Which parameters should I know? Is there any formula to go from particles number to...

03 September 2014 7,009 19 View

How can I use AIBN (2,2′-Azobis(2-methylpropionitrile) for cross linking?

I would like to know how you guys use AIBN for cross linking? Should I just mix a certain amount of the monomer and AIBN and let them interact at a specific temperature? What are the tips for the...

10 April 2014 8,299 4 View

What are the reasons that polymers (not conducting) interact with double stranded DNA while not interacings with single stranded DNA?

Polymer and DNA interaction

09 January 2014 7,768 3 View

How can we understand if a polymer is conducting or not?

I have a polymer which hasn't been studied before. I did some cyclic voltammetry studies to evaluate it has oxidation or reduction potential or not. I found that this polymer has oxidation polymer...

09 October 2013 7,094 11 View

Best hybridization buffer for DNA Hybridization?

I would like to use hybridization between a probe and target DNA. We usually use PBS Buffer as a hybridization buffer. I want to try another solution. Any recommendations?

01 October 2013 1,838 30 View

Can someone advise on how to calculate detection limit of an experiment related to probe concentration? Is there any formula?

X axis: diffferent concentrations of DNA y AXİS: current I have avarage standart deviation value: 0.29 My equation: y=0,95 x+0.4467 R2=0,9928 Also, what is the difference between sensitivity...

22 July 2013 2,728 6 View

What is the PCR amplification of specific alleles (PASA) method?

For PASA Method, specific primers are generally used. What is the point of designing primer? What should be considered when designing primers?

25 February 2013 1,580 1 View

What is the best cardiomyocytes: cardiofibroblast co-culture ratio for 2D experiments?

Also, what is the suitable concentration of cardiomyocytes to take an effective beating with 2D samples?

10 February 2013 5,909 1 View

Does anyone know about how long cardiomyocytes could wait in the incubator after isolation?

After the isolation of cardiomyocytes from the neonatal rat heart, how long they can survive?

07 January 2013 8,411 2 View