Dear all,
I am trying to locate the protein reacting sites after SBED modification.
Generally, protein A was firstly modified by SBED through NHS/NH2. Then, incubated with protein B and irradiated by UV. DTT/IAA was used to open disulfide bond and alkylate. After enrichment, protein B was separated by SDS-PAGE, and further analysed by MS/MS.
I tried set 606.2532 as a variable modification for all AA or only 4 kinds of AA (G R K and L), and found nothing by PD. Does anyone happen to know a software can do this analysis? I will greatly appreciate you kindly help.
Thank you.
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