How many targets can bind to one aptamer?

Congcong Lu @Congcong_Lu

11 November 2016 0 155 Report

Just curious about this question.

Does one aptamer can only bind to one target protein?

Is there a number showing the binding capability of one aptamer?

Thank you.

Badges
Science topic

More Congcong Lu's questions See All

Does anyone know a software could analyse photo-crosslinked MS/MS data?

Dear all, I am trying to locate the protein reacting sites after SBED modification. Generally, protein A was firstly modified by SBED through NHS/NH2. Then, incubated with protein B and irradiated...

07 August 2016 4,877 0 View

Does anyone familiar with desalting of oligonucleotide samples using ZipTip?

How to desalt DNA samples with ZipTip pipette (Millipore, C18)? Same with peptides? The user guide provided by Millipore said that information on concentrating and desalting oligonucleotides...

09 October 2014 6,507 2 View

How can I destroy non-covalent interactions between proteins?

NaCl could destroy the electrostatic interactions. HAc could damage the hydrogen bond between proteins. Is this right? How to destroy non-covalent interactions between proteins?

07 August 2014 6,732 5 View

Which matrix is better for detecting the molecular weight of DNA by MALDI-MS?

I have three DNA samples about 5.5, 9.5 and 16.5 KDa, respectively. Now, I want to detect them by MALDI-MS. Did anyone know which matrix is better for DNA detecting, 3-HPA, 2,4,6-THAP or 3,4-DABP?...

06 July 2014 8,199 14 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

How to avoid background signal in screen printed electrode?

Hi, I am doing aptamer based electrochemical biosensor. First, I cleaned screen printed gold electrode using 0.5M H2SO4. Then, immobilized aptamer followed by blocking using 2mM MCH. I use...

06 July 2024 9,852 1 View