Does anyone know a software could analyse photo-crosslinked MS/MS data?

Dear all, I am trying to locate the protein reacting sites after SBED modification. Generally, protein A was firstly modified by SBED through NHS/NH2. Then, incubated with protein B and irradiated...

07 August 2016 4,833 0 View

Does anyone familiar with desalting of oligonucleotide samples using ZipTip?

How to desalt DNA samples with ZipTip pipette (Millipore, C18)? Same with peptides? The user guide provided by Millipore said that information on concentrating and desalting oligonucleotides...

09 October 2014 6,444 2 View

How can I destroy non-covalent interactions between proteins?

NaCl could destroy the electrostatic interactions. HAc could damage the hydrogen bond between proteins. Is this right? How to destroy non-covalent interactions between proteins?

07 August 2014 6,691 5 View

Which matrix is better for detecting the molecular weight of DNA by MALDI-MS?

I have three DNA samples about 5.5, 9.5 and 16.5 KDa, respectively. Now, I want to detect them by MALDI-MS. Did anyone know which matrix is better for DNA detecting, 3-HPA, 2,4,6-THAP or 3,4-DABP?...

06 July 2014 8,155 14 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

How to find a particular aptamer complementary sequence?

I can find several papers that give me a guide for choosing a suitable aptamer sequence 1. But how to find a property aptamer sequence 2(partially complementary to sequence 1)?

09 February 2021 3,511 1 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View