Does anyone know a set of primers for Trypanosoma vivax?

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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Juan David Ramírez

Hello Luiz

Those reported by Masake are the most sensitive ones and work well.

R. A. Masake, P. A. Majiwa, S. K. Moloo, J. M. Makau, J. T. Njuguna,

V. M. Nantulya, “Sensitive and Specific Detection of Trypanosoma vivax Using the Polymerase Chain Reaction,” Experimental Parasitology, Vol. 85, No. 2, 1997, pp. 193-205. doi:10.1006/expr.1996.4124

I hope that works

Luiz Daniel de Barros

Thank you Dr. Ramírez.

I will try to use those.

Armando Reyna-Bello

Totally agree with Dr. Juan David. We use the priemer decribed by Nantulya in our lab

Armando Reyna