Hello Luiz

Those reported by Masake are the most sensitive ones and work well.

R. A. Masake, P. A. Majiwa, S. K. Moloo, J. M. Makau, J. T. Njuguna, 

V. M. Nantulya, “Sensitive and Specific Detection of Trypanosoma vivax Using the Polymerase Chain Reaction,” Experimental Parasitology, Vol. 85, No. 2, 1997, pp. 193-205. doi:10.1006/expr.1996.4124 

I hope that works

Thank you Dr. Ramírez. 

I will try to use those.

Totally agree with Dr. Juan David. We use the priemer decribed by Nantulya in our lab

Armando Reyna