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Hi! This protocol has been used in my lab to handle healthy rat liver samples (see below). But now, i'm going to start a project for quantifying membrane proteins in healthy and diseased human...
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Hi! I'm trying to express a human protein (GST-NTCP) in E.coli (BL21 cells). The protein is cloned in pGEX6P-1 vector and is 64KDa in size. After DNA cloning, the plasmid's sequence was correct so...
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