I know protocols of Chromatin Immunprecipitation, but I want to do a previous test befor doing ChIP
My protein of interest possess a HLH-similar sequence, but it is not a consensus HLH-sequence.
EMSA is quick. This will give accurate results also.
Thank you! I will give it try :)
Kind regards
Who is going to the 10th Circulating Nucleic Acids in Plasma and Serum Symposium from 20-22 of September? Maybe we can proceed in networking and exchange of ideas, data and experiences? Looking...
05 June 2017 7,739 0 View
Hello, I am looking for an rel. easy Explanation/Definition to the Linear Dynamic Range term in regard to nanostring Technologies. Pls, could someone give me an short Explanation? Thank you very...
09 October 2016 3,975 1 View
I just know from Southern Blotting, that ssDNA binds to it
08 September 2013 4,269 1 View
ctDNA is a promising biomarker and part of liquid biopsy (next to CTCs, cfmiRNA etc.). Analyizing ctDNA could help to detected e.g. pre-clinical cancer or supports diagnostic procedures. However,...
01 January 1970 9,700 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View