Everyone is detecting tocopherols of olive oil with HPLC and an FD detector. Does anyone know a process for detecting tocopherols with an HPLC and a UV-VIS detector?
Or this is better
http://web.campbell.edu/faculty/bryan/CHEM441/JournalArticles/cereal.pdf
Here's a reference for saponifying the fatty acid lipids so as to enrich the tocopherols, as mentioned in Narong's first reference:
Adidi, S.L. J. of Chrom. A., 2000, 881, 197
Basically, they saponify the fatty acid esters and extract what remains into ether. The ether extract could potentially be used as you describe.
Jack's paper is good idea to separate fatty acids from non-saponify vitamin E because the UV detector is not as selective and it will clean the chromatogram better and you also concentrate to get the desired detection limit.
You can read these papers:
P Manzi, G Panfili, M Esti and L Pizzoferrato
J Sci Food Agric 1998, 77, 115-120
Natural Antioxidants in the Unsaponifable Fraction of Virgin Olive Oils From Different
Cultivars
M Esti,L Cinquanta, G Panfili, P Manzi , S Marconi and L Pizzoferrato
Effects of variety and olive ripeness on nutritional quality and oxidative stability of extra virgin olive oils
Food, Agriculture & Environment Vol.2 (2) : 129-134. 2004 WFL Publisher Science and Technology
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