Does anyone know a method to specifically enrich nucleoli beyond isolating nuclei?

Kristopher Charles Hite @Kristopher-Hite

11 January 2013 1 3K Report

I'm trying to monitor the expression levels of a single component of a chromatin remodeling complex at work in the nucleolus of Arabidopsis. Any tricks out there for enriching this tiny organelle? Sucrose gradient?

Pooneh Ardestani

I have never done that but I think Lamond lab (University of Dundee) is very good at that. you can use their protocols or contact them. http://www.lamondlab.com/f7nucleolarprotocol.htm

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