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Questions related from Pooneh Ardestani
My protein of interest is in the cytosolic fraction of mouse amygdala (using an NE-PER kit) in western blot but in IHC it is mostly nuclear. Any ideas? Any idea for fractionation of frozen tissue...
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Does it need any further preparation, or should we be able to differentiate dense and loose chromatin in TEM? I have stained HeLa cells for a protein which should be associated with euchromatin. I...
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