Is a multivariate linear mixed model appropriate to analyse differences over time?

Say I have a data set of 32 study participants. The dependent variables are two different physiological reactions to stimuli. These are repeated measures, recorded over 16 trials. There are two...

13 December 2020 7,218 3 View

Calculating effect sizes without knowing the sample size by subgroups?

Hello! I am calculating effect sizes for a research synthesis of the literature (e.g. Hedges' g), but many of the original publications do not provide the sample size (N) by subgroups. I see...

13 September 2020 7,587 3 View

Does anyone have a user manual for the Cahn DCA-322 Dynamic Contact Angle Analyser?

Our instrument is from about 1998 and we no longer have the manual. The Help function in the program needs to connect to the internet, but we have to keep the computer offline, therefore the help...

29 May 2019 6,109 3 View

Does anyone know of a dataset for seismic activity for Europe?

29 March 2019 4,501 3 View

How can I change my entry regarding my book?

I mistakenly wrote that my book would be open access. This is not the case and needs to be changed. Presumably this means that I cannot have reviews of it either?

07 March 2019 1,442 1 View

A problem with generation of lentiviral particles of a large gene (4kb)?

I am trying to generate lentiviral particles from a insert (4Kb) cloned into a lentiviral vector (pWPI, 11Kb) and I am having some issues. I transfected HEK 293T cells with the pMD2G, pPAX and our...

02 August 2018 5,170 3 View

Does anyone know how to calculate DNA bending angle with curves+ program?

I am working with DNA-Protein complexes and I tried to determinate it with x3dna but i had some problems with the vectors, so i read that other used program is curves+. Has anyone worked with this...

27 March 2018 277 1 View

What is the future of labor law in a future world without work?

Labor law is predominantly carried out from the domestic perspective of the countries through their legislation, accepting only some international (often disregarded) legislative...

23 March 2018 473 6 View

Hello everyone, I am wondering whether just from the EEG raw data alone you can identify where on the scalp the electrode is positioned?

In more detail, we are interested whether we can distinguish the HEOG electrode from the F7 electrode next to it? Thank you very much in advance and all the best, Miriam

24 September 2017 149 1 View

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13 June 2017 7,057 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View